MIT researchers map the energy transition’s effects on jobs

A new analysis by MIT researchers shows the places in the U.S. where jobs are most linked to fossil fuels. The research could help policymakers better identify and support areas affected over time by a switch to renewable energy.

While many of the places most potentially affected have intensive drilling and mining operations, the study also measures how areas reliant on other industries, such as heavy manufacturing, could experience changes. The research examines the entire U.S. on a county-by-county level.

“Our result is that you see a higher carbon footprint for jobs in places that drill for oil, mine for coal, and drill for natural gas, which is evident in our maps,” says Christopher Knittel, an economist at the MIT Sloan School of Management and co-author of a new paper detailing the findings. “But you also see high carbon footprints in areas where we do a lot of manufacturing, which is more likely to be missed by policymakers when examining how the transition to a zero-carbon economy will affect jobs.”

So, while certain U.S. areas known for fossil-fuel production would certainly be affected — including west Texas, the Powder River Basin of Montana and Wyoming, parts of Appalachia, and more — a variety of industrial areas in the Great Plains and Midwest could see employment evolve as well.

The paper, “Assessing the distribution of employment vulnerability to the energy transition using employment carbon footprints,” is published this week in Proceedings of the National Academy of Sciences. The authors are Kailin Graham, a master’s student in MIT’s Technology and Policy Program and graduate research assistant at MIT’s Center for Energy and Environmental Policy Research; and Knittel, who is the George P. Shultz Professor at MIT Sloan.

“Our results are unique in that we cover close to the entire U.S. economy and consider the impacts on places that produce fossil fuels but also on places that consume a lot of coal, oil, or natural gas for energy,” says Graham. “This approach gives us a much more complete picture of where communities might be affected and how support should be targeted.”

Adjusting the targets

The current study stems from prior research Knittel has conducted, measuring carbon footprints at the household level across the U.S. The new project takes a conceptually related approach, but for jobs in a given county. To conduct the study, the researchers used several data sources measuring energy consumption by businesses, as well as detailed employment data from the U.S. Census Bureau.

The study takes advantage of changes in energy supply and demand over time to estimate how strongly a full range of jobs, not just those in energy production, are linked to use of fossil fuels. The sectors accounted for in the study comprise 86 percent of U.S. employment, and 94 percent of U.S. emissions apart from the transportation sector.

The Inflation Reduction Act, passed by Congress and signed into law by President Joe Biden in August 2022, is the first federal legislation seeking to provide an economic buffer for places affected by the transition away from fossil fuels. The act provides expanded tax credits for economic projects located in “energy community” areas — defined largely as places with high fossil-fuel industry employment or tax revenue and with high unemployment. Areas with recently closed or downsized coal mines or power plants also qualify.

Graham and Knittel measured the “employment carbon footprint” (ECF) of each county in the U.S., producing new results. Out of more than 3,000 counties in the U.S., the researchers found that 124 are at the 90th percentile or above in ECF terms, while not qualifying for Inflation Reduction Act assistance. Another 79 counties are eligible for Inflation Reduction Act assistance, while being in the bottom 20 percent nationally in ECF terms.

Those may not seem like colossal differences, but the findings identify real communities potentially being left out of federal policy, and highlight the need for new targeting of such programs. The research by Graham and Knittel offers a precise way to assess the industrial composition of U.S. counties, potentially helping to target economic assistance programs.

“The impact on jobs of the energy transition is not just going to be where oil and natural gas are drilled, it’s going to be all the way up and down the value chain of things we make in the U.S.,” Knittel says. “That’s a more extensive, but still focused, problem.”

Graham adds: “It’s important that policymakers understand these economy-wide employment impacts. Our aim in providing these data is to help policymakers incorporate these considerations into future policies like the Inflation Reduction Act.”

Adapting policy

Graham and Knittel are still evaluating what the best policy measures might be to help places in the U.S. adapt to a move away from fossil fuels.

“What we haven’t necessarily closed the loop on is the right way to build a policy that takes account of these factors,” Knittel says. “The Inflation Reduction Act is the first policy to think about a [fair] energy transition because it has these subsidies for energy-dependent counties.” But given enough political backing, there may be room for additional policy measures in this area.

One thing clearly showing through in the study’s data is that many U.S. counties are in a variety of situations, so there may be no one-size-fits-all approach to encouraging economic growth while making a switch to clean energy. What suits west Texas or Wyoming best may not work for more manufacturing-based local economies. And even among primary energy-production areas, there may be distinctions, among those drilling for oil or natural gas and those producing coal, based on the particular economics of those fuels. The study includes in-depth data about each county, characterizing its industrial portfolio, which may help tailor approaches to a range of economic situations.

“The next step is using this data more specifically to design policies to protect these communities,” Knittel says.

MIT-led team receives funding to pursue new treatments for metabolic disease

A team of MIT researchers will lead a $65.67 million effort, awarded by the U.S. Advanced Research Projects Agency for Health (ARPA-H), to develop ingestible devices that may one day be used to treat diabetes, obesity, and other conditions through oral delivery of mRNA. Such devices could potentially be deployed for needle-free delivery of mRNA vaccines as well.

The five-year project also aims to develop electroceuticals, a new form of ingestible therapies based on electrical stimulation of the body’s own hormones and neural signaling. If successful, this approach could lead to new treatments for a variety of metabolic disorders.

“We know that the oral route is generally the preferred route of administration for both patients and health care providers,” says Giovanni Traverso, an associate professor of mechanical engineering at MIT and a gastroenterologist at Brigham and Women’s Hospital. “Our primary focus is on disorders of metabolism because they affect a lot of people, but the platforms we’re developing could be applied very broadly.”

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A team of MIT researchers will lead an effort to develop new ingestible devices that may one day be used to treat diabetes, obesity, and other conditions through oral delivery of mRNA or electrical stimulation to the GI tract.

Traverso is the principal investigator for the project, which also includes Robert Langer, MIT Institute Professor, and Anantha Chandrakasan, dean of the MIT School of Engineering and the Vannevar Bush Professor of Electrical Engineering and Computer Science. As part of the project, the MIT team will collaborate with investigators from Brigham and Women’s Hospital, New York University, and the University of Colorado School of Medicine.

Over the past several years, Traverso’s and Langer’s labs have designed many types of ingestible devices that can deliver drugs to the GI tract. This approach could be especially useful for protein drugs and nucleic acids, which typically can’t be given orally because they break down in the acidic environment of the digestive tract.

Messenger RNA has already proven useful as a vaccine, directing cells to produce fragments of viral proteins that trigger an immune response. Delivering mRNA to cells also holds potential to stimulate production of therapeutic molecules to treat a variety of diseases. In this project, the researchers plan to focus on metabolic diseases such as diabetes.

“What mRNA can do is enable the potential for dosing therapies that are very difficult to dose today, or provide longer-term coverage by essentially creating an internal factory that produces a therapy for a prolonged period,” Traverso says.

In the mRNA portion of the project, the research team intends to identify lipid and polymer nanoparticle formulations that can most effectively deliver mRNA to cells, using machine learning to help identify the best candidates. They will also develop and test ingestible devices to carry the mRNA-nanoparticle payload, with the goal of running a clinical trial in the final year of the five-year project.

The work will build on research that Traverso’s lab has already begun. In 2022, Traverso and his colleagues reported that they could deliver mRNA in capsules that inject mRNA-nanoparticle complexes into the lining of the stomach.

The other branch of the project will focus on ingestible devices that can deliver a small electrical current to the lining of the stomach. In a study published last year, Traverso’s lab demonstrated this approach for the first time, using a capsule coated with electrodes that apply an electrical current to cells of the stomach. In animal studies, they found that this stimulation boosted production of ghrelin, a hormone that stimulates appetite.

Traverso envisions that this type of treatment could potentially replace or complement some of the existing drugs used to prevent nausea and stimulate appetite in people with anorexia or cachexia (loss of body mass that can occur in patients with cancer or other chronic diseases). The researchers also hope to develop ways to stimulate production of GLP-1, a hormone that is used to help manage diabetes and promote weight loss.

“What this approach starts to do is potentially maximize our ability to treat disease without administering a new drug, but instead by simply modulating the body’s own systems through electrical stimulation,” Traverso says.

At MIT, Langer will help to develop nanoparticles for mRNA delivery, and Chandrakasan will work on ways to reduce energy consumption and miniaturize the electronic functions of the capsules, including secure communication, stimulation, and power generation.

The Brigham and Women’s Hospital’s portion of the project will be co-led by Traverso, Ameya Kirtane, Jason Li, and Peter Chai, who will amplify efforts on the formulation and stabilization of the mRNA nanoparticles, engineering of the ingestible devices, and running of clinical trials. At NYU, the effort will be led by assistant professor of bioengineering Khalil Ramadi SM ’16, PhD ’19, focusing on biological characterization of the effects of electrical stimulation. Researchers at the University of Colorado, led by Matthew Wynia and Eric G. Campbell of the CU Center for Bioethics and Humanities, will focus on exploring the ethical dimensions and public perceptions of these types of biomedical interventions.

“We felt like we had an opportunity here not only to do fundamental engineering science and early-stage clinical trials, but also to start to understand the data behind some of the ethical implications and public perceptions of these technologies through this broad collaboration,” Traverso says.

The project described here is supported by ARPA-H under award number D24AC00040-00. The content of this announcement does not necessarily represent the official views of the Advanced Research Projects Agency for Health.

ATOMOS UPDATES – The Best Just Got Better – Videoguys

Tune in to today’s episode of Videoguys Live as Jim delves into the latest offerings from Atomos Monitors and Recorders, spotlighting the innovative Connect line and exciting promotional deals. Stay updated on cutting-edge technology and discover how these products can elevate your filmmaking and video production endeavors. Don’t miss out on exclusive insights and special offers

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TURBO CHARGE NINJA!
Special Atomos Connect Promotion for just $99 through 3/31/24 means
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Elevate your Ninja with Atomos Connect for only $99! Atomos Connect is the perfect match for any Ninja, Ninja Ultra, Ninja V and Ninja V+ user.

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Atomos Cloud Studio
The centralized gateway between Atomos’ network-connected hardware solutions and a host of online services

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GET CONNECTED: 6 Months FREE Camera to Cloud Plan
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With every new purchase of Atomos Connect, Zato Connect, Shogun, or Shogun Ultra, you will receive a complimentary six-month Camera to Cloud paid plan on Atomos Cloud Studio (valued at $15/month).

This plan grants access to a range of premium features, including selectable proxy and stream resolutions (720p, 1080p & 4K), customizable bit depth and bitrate settings, and seamless uploading of C2C files during recording, optimizing workflow efficiency. Users can choose between Atomos Edit, Frame.io, and Sony Ci Media Cloud as their preferred destination, ensuring seamless compatibility with existing workflows and collaboration tools.


Atomos is thrilled to unveil today the complimentary user interface upgrade to AtomOS 11 for Ninja V/V+ users, along with optional paid feature packs designed to elevate their Ninja experience. Access to the latest Ninja and Ninja Ultra new features has been a consistent request from their Ninja V/V+ users since the introduction of the new Ninja Series. In line with their dedication to their customers, these new feature packs not only add value to Ninja V/V+ but also reaffirm their commitment to delivering exceptional service.

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For a limited period only, the Ninja V Feature Pack is available for $79 and the Ninja V+ Feature Pack for $129 for purchase at my.atomos.com.


In addition, Atomos is excited to reintroduce the 3-Year Free Warranty on all purchases after product registration.

New Epiphan Pearl Nexus Certified by Panopto! – Videoguys

Panopto, a prominent global provider of video management solutions catering to both workforce training and higher education, has unveiled a new certified device, the Epiphan Pearl Nexus. This announcement signals a significant collaboration between the two companies aimed at revolutionizing video capture experiences. The Pearl Nexus device seamlessly integrates with Panopto’s renowned video management platform, promising users a superior and automated video capture experience.

Epiphan’s Pearl Nexus is equipped with advanced recording and streaming capabilities, supporting up to three channels of 1080p video. Its versatility extends to a wide range of video inputs, including SDI, HDMI, USB, SRT, and NDI, along with professional audio inputs such as XLR, USB, and 3.5 mm. This flexibility ensures compatibility with modern AV workflows, offering users creative controls and cloud management tools to streamline their operations. Additionally, features like a 1 TB SSD drive, CMS integrations, and automated file management underscore the device’s commitment to secure content recording and precise delivery.

The partnership between Panopto and Epiphan signifies a commitment to enhancing online learning experiences for both educational institutions and commercial enterprises. By leveraging Epiphan’s expertise in hardware-based lecture capture at scale and Panopto’s robust video management platform, the collaboration aims to meet the growing demand for classroom capture solutions. Through this joint effort, the companies strive to empower faculty, administrators, and students alike with an automated and integrated video capture solution, ultimately enhancing knowledge sharing, remote recording capabilities, and content delivery across diverse environments.

Read the full Press Release from Epiphan HERE

Kiloview N40 NDI Success Story – Videoguys

In February 2021, Toyama Prefecture in Japan unveiled its ambitious growth strategy, aiming to leverage its unique resources for economic development. To realize this vision, they convened the Toyama Growth Strategy Conference, a gathering of experts across various sectors to formulate a comprehensive strategy encompassing industrial promotion, innovation, talent development, and regional resource utilization.

To enhance outreach, the “Shiawaseru (Creating Happiness). Toyama” event was organized in March 2022. Led by Mr. Kotaro Ebitani of EverT, the event featured a hybrid format blending physical and online components across multiple venues, including the Toyama International Conference Center.

However, the setup posed significant challenges, with the cumbersome arrangement of cables hindering efficiency and flexibility. Recognizing the need for a streamlined solution, Mr. Yuto Izumi of KAN-NARI CO., LTD AVC Division proposed integrating NDI technology, known for its simplicity and efficiency in video transmission.

The introduction of Kiloview’s N40 NDI converter revolutionized the event setup. Its user-friendly interface and robust features, including bi-directional conversion and support for 4K resolution, seamlessly integrated with existing equipment. By transmitting HDMI signals into NDI, the N40 facilitated smooth communication with the TriCaster 2 Elite, the main switcher for live streaming.

The outcome was a resounding success. The “Shiawaseru. Toyama” event effectively communicated Toyama Prefecture’s growth plans, fostering economic development and regional prosperity. Notably, the flexibility of NDI technology opens avenues for future hybrid events, aligning with the evolving landscape of online and offline engagements.

Moreover, the affordability and performance of Kiloview’s N40 set it apart from competitors, offering a cost-effective solution without compromising quality. Its compatibility with a range of IP protocols, primarily NDI, makes it an ideal choice for organizations seeking to embrace IP workflows.

Looking ahead, the collaboration between industry professionals and innovative technologies like the N40 promises to drive further growth and innovation, positioning Toyama Prefecture at the forefront of economic development in Japan.

Read the full Case Study from Kiloview HERE

Remembering MIT Copytech Director Casey Harrington

Casey Harrington, who led MIT Copytech’s recovery from pandemic-era disruptions and built close friendships across campus, passed away unexpectedly on Jan. 13. He was 49.

Copytech’s director since 2022, Harrington modernized the department’s equipment and services to improve its financial outlook, and led his staff with a personal touch.

“Casey was beloved by our team,” says Alfred Ironside, MIT’s vice president for communications. “He was a great manager, had a vision for the future, spent time with his co-workers, cared for them, and loved MIT. He turned around Copytech’s fortunes, too, setting it on an upward trajectory that reflected his wonderful abilities as a leader. His loss is enormous — for his wife, his children, his family, and for us.”

Although Harrington’s time at MIT was brief, he left a lasting impression on his team at Copytech and the people he worked with every day.

“Casey was a great leader, with a rare combination of vision, approachability, and genuine care and appreciation for our team,” Financial Officer Suha Bekdash says. “Since he joined two years ago, he made an immediate impact steering Copytech toward a more successful future after the pandemic.”

For those who knew Harrington well, he will be remembered for his numerous “Casey-isms,” which included “Be grateful” and “Do the next right thing.”

“I want his legacy to be, ‘Do the next right thing,’” his wife Marilyn Harrington says. “Coming to MIT was the next right thing for our family, and he always did the next right thing for our family. There was no way for us to predict this tragedy. He did the next right thing for his staff and for MIT leadership. He always did his best. He left this world very loved, very respected, and he left a hole that will never be filled.”

Leading Copytech by building friendships

Harrington came to MIT with big shoes to fill. His predecessor, Steven Dimond, had worked at Copytech for 50 years. On top of that, Copytech was emerging from a pandemic that caused business to stall as people left campus and more events went online.

“That had been an extremely difficult number of years for Copytech, both financially and also just for the morale of people,” says Danyel Barnard, MIT’s executive director of digital, brand, and internal communications. “He took a job that was a challenge. We needed someone who could come in and help turn things around. He was really excited about the opportunity, and about being at MIT, and he was eager to lead the organization through those challenges.”

Harrington came to MIT with deep expertise about the print industry. Prior to MIT, he had worked at large private companies, global health care groups, and Vanderbilt University.

“He could take the transcript of a book and say, ‘It needs to be this size book print with this size paper and this many pages,’” Marilyn Harrington recalls.

At MIT, he hit the ground running by working out new service contracts and pitching ideas for new equipment, including a new, large-format printing machine.

“In his two years here, he did amazing things with the operation,” says Barnard. “He led a big turnaround financially, a lot of which I credit to his management and leadership. He had great foresight and truly exceeded expectations.”

Most importantly, Harrington established personal connections with the Copytech team and his colleagues across the Institute.

“Casey was a great leader,” Administrative Assistant Taj Dickson says. “He loved Copytech and he loved MIT. When he came, he fit right in with us. People have been in Copytech for so long, and when they leave there’s always trepidation that the new person is not going to understand how to deal with the department. But he came in and figured it out, and the transition was really smooth because of his emotional intelligence as a leader. I think that was one of his hallmarks: He was a very emotionally intelligent leader.”

It was perhaps an unlikely match. Harrington’s southern accent was a stark contrast with many of the Bostonians in Copytech. But their different backgrounds served as a conversation piece more than a point of difference.

“The staff universally loved him,” Barnard says. “He was a perfect fit and a perfect leader for them. He really cared about them, and that is so important at Copytech, where they consider themselves a family.”

His wife describes MIT as Harrington’s “dream job” and says he was grateful to Copytech’s staff for embracing him.

“He left MIT in a better place than he found it because of the support he got from the team and from MIT leadership,” she says.

A strong leader

Harrington was born in Nashville and was a proud graduate of the University of Tennessee at Knoxville. He was also deeply devoted to his family. He met Marilyn when he was 13, and their friendship blossomed into a 27-year marriage.

Casey moved to Boston while his family figured out where their youngest child would attend high school, but they made a point to see each other as much as possible — Marilyn estimates they spent well over 400 days together in his first two years living in Boston.

“Once, I texted him that I wasn’t doing well and I needed to see him, and he was on my doorstep four hours later,” Marilyn recalls. “He didn’t even have a bag, only his laptop. That was the kind of person he was.”

“You could really have honest conversations with Casey,” Dickson says. “He would have no problem talking about his experiences, good and bad, and it was up to you to find the lesson in those stories.”

When news of Harrington’s passing got around MIT, Barnard heard from people in disparate departments who she didn’t even realize knew Harrington explaining they had become friends.

“He found ways to connect with people,” Barnard says. “I was amazed by his reach.”

In honor of Harrington and in a nod to his love for University of Tennessee football, the Copytech team wore orange shirts and blue jeans to work on Jan. 29. They say they’ll continue to honor Harrington through their work.

“He was a strong leader who was full of life, and he still had so much to offer Copytech,” Dickson says. “His ability to communicate, his unique sense of humor, and his love for our department were just a few of the highlights that made Casey shine.”

MADMEC winner creates “temporary tattoos” for T-shirts

Have you ever gotten a free T-shirt at an event that you never wear? What about a music or sports-themed shirt you wear to one event and then lose interest in entirely? Such one-off T-shirts — and the waste and pollution associated with them — are an unfortunately common part of our society.

But what if you could change the designs on shirts after each use? The winners of this year’s MADMEC competition developed biodegradable “temporary tattoos” for T-shirts to make one-wear clothing more sustainable.

Members of the winning team, called Me-Shirts, got their inspiration from the MADMEC event itself, which ordinarily makes a different T-shirt each year.

“If you think about all the textile waste that’s produced for all these shirts, it’s insane,” team member and PhD candidate Isabella Caruso said in the winning presentation. “The main markets we are trying to address are for one-time T-shirts and custom T-shirts.”

The problem is a big one. According to the team, the custom T-shirt market is a $4.3 billion industry. That doesn’t include trends like fast fashion that contribute to the 17 million tons of textile waste produced each year.

“Our proposed solution is a temporary shirt tattoo made from biodegradable, nontoxic materials,” Caruso explained. “We wanted designs that are fully removable through washing, so that you can wear your T-shirt for your one-time event and then get a nice white T-shirt back afterward.”

The team’s scalable design process mixes three simple ingredients: potato starch, glycerin, and water. The design can be imprinted on the shirt temporarily through ironing.

The Me-Shirt team, which earned $10,000 with the win, plans to continue exploring material combinations to make the design more flexible and easier for people to apply at home. Future iterations could allow users to decide if they want the design to stay on the shirt during washes based on the settings of the washing machine.

Hosted by MIT’s Department of Materials Science and Engineering (DMSE), the competition was the culmination of team projects that began in the fall and included a series of design challenges throughout the semester. Each team received guidance, access to equipment and labs, and up to $1,000 in funding to build and test their prototypes.

“The main goal is that they gained some confidence in their ability to design and build devices and platforms that are different from their normal experiences,” Mike Tarkanian, a senior lecturer in DMSE and coordinator of MADMEC, said at the event. “If it’s a departure from their normal research and coursework activities that’s a win, I think, to make them better engineers.”

The second-place, $6,000 prize went to Alkalyne, which is creating a carbon-neutral polymer for petrochemical production. The company is developing approaches for using electricity and inorganic carbon to generate a high-energy hydrocarbon precursor. If developed using renewable energy, the approach could be used to achieve carbon negative petrochemical production.

“A lot of our research, and a lot of the research around MIT in general, has to do with sustainability, so we wanted to try an angle that we think looks promising but doesn’t seem to be investigated enough,” PhD candidate Christopher Mallia explained.

The third-place prize went to Microbeco, which is exploring the use of microbial fuel cells for continuous water quality monitoring. Microbes have been proposed as a way to detect and measure contaminants in water for decades, but the team believes the varying responses of microbes to different contaminants has limited the effectiveness of the approach.

To overcome that problem, the team is working to isolate microbial strains that respond more regularly to specific contaminants.

Overall, Tarkanian believes this year’s program was a success not only because of the final results presented at the competition, but because of the experience the students got along the way using equipment like laser cutters, 3D printers, and soldering irons. Many participants said they had never used that type of equipment before. They also said by working to build physical prototypes, the program helped make their coursework come to life.

“It was a chance to try something new by applying my skills to a different environment,” PhD candidate Zachary Adams said. “I can see a lot of the concepts I learn in my classes through this work.”

Scientists develop a low-cost device to make cell therapy safer

A tiny device built by scientists at MIT and the Singapore-MIT Alliance for Research and Technology could be used to improve the safety and effectiveness of cell therapy treatments for patients suffering from spinal cord injuries.

In cell therapy, clinicians create what are known as induced pluripotent stem cells by reprogramming some skin or blood cells taken from a patient. To treat a spinal cord injury, they would coax these pluripotent stem cells to become progenitor cells, which are destined to differentiate into spinal cord cells. These progenitors are then transplanted back into the patient.

These new cells can regenerate part of the injured spinal cord. However, pluripotent stem cells that don’t fully change into progenitors can form tumors.

This research team developed a microfluidic cell sorter that can remove about half of the undifferentiated cells — those that can potentially become tumors — in a batch, without causing any damage to the fully-formed progenitor cells.

The high-throughput device, which doesn’t require special chemicals, can sort more than 3 million cells per minute. In addition, the researchers have shown that chaining many devices together can sort more than 500 million cells per minute, making this a more viable method to someday improve the safety of cell therapy treatments.

Plus, the plastic chip that contains the microfluidic cell sorter can be mass-produced in a factory at very low cost, so the device would be easier to implement at scale.

“Even if you have a life-saving cell therapy that is doing wonders for patients, if you cannot manufacture it cost-effectively, reliably, and safely, then its impact might be limited. Our team is passionate about that problem — we want to make these therapies more reliable and easily accessible,” says Jongyoon Han, an MIT professor of electrical engineering and computer science and of biological engineering, a member of the Research Laboratory of Electronics (RLE), and co-lead principal investigator of the CAMP (Critical Analytics for Manufacturing Personalized Medicine) research group at the Singapore-MIT Alliance for Research and Technology (SMART).

Han is joined on the paper by co-senior author Sing Yian Chew, professor of chemistry, chemical engineering, and biotechnology at the Lee Kong Chian School of Medicine and Materials Science and Engineering at Nanyang Technological University in Singapore and a CAMP principal investigator; co-lead authors Tan Dai Nguyen, a CAMP researcher; Wai Hon Chooi, a senior research fellow at the Singapore Agency for Science, Technology, and Research (A*STAR); and Hyungkook Jeon, an MIT postdoc; as well as others at NTU and A*STAR. The research appears today in Stem Cells Translational Medicine.

Reducing risk

The cancer risk posed by undifferentiated induced pluripotent stem cells remains one of the most pressing challenges in this type of cell therapy.

“Even if you have a very small population of cells that are not fully differentiated, they could still turn into cancer-like cells,” Han adds.

Clinicians and researchers often seek to identify and remove these cells by looking for certain markers on their surfaces, but so far researchers have not been able to find a marker that is specific to these undifferentiated cells. Other methods use chemicals to selectively destroy these cells, yet the chemical treatment techniques may be harmful to the differentiated cells.

The high-throughput microfluidic sorter, which can sort cells based on size, had been previously developed by the CAMP team after more than a decade of work. It has been previously used for sorting immune cells and mesenchymal stromal cells (another type of stem cell), and now the team is expanding its use to other stem cell types, such as induced pluripotent stem cells, Han says.

“We are interested in regenerative strategies to enhance tissue repair after spinal cord injuries, as these conditions lead to devasting functional impairment. Unfortunately, there is currently no effective regenerative treatment approach for spinal cord injuries,” Chew says. “Spinal cord progenitor cells derived from pluripotent stem cells hold great promise, since they can generate all cell types found within the spinal cord to restore tissue structure and function. To be able to effectively utilize these cells, the first step would be to ensure their safety, which is the aim of our work.”

The team discovered that pluripotent stem cells tend to be larger than the progenitors derived from them. It is hypothesized that before a pluripotent stem cell differentiates, its nucleus contains a large number of genes that haven’t been turned off, or suppressed. As it differentiates for a specific function, the cell suppresses many genes it will no longer need, significantly shrinking the nucleus.

The microfluidic device leverages this size difference to sort the cells.

Spiral sorting

Microfluidic channels in the quarter-sized plastic chip form an inlet, a spiral, and four outlets that output cells of different sizes. As the cells are forced through the spiral at very high speeds, various forces, including centrifugal forces, act on the cells. These forces counteract to focus the cells in a certain location in the fluid stream. This focusing point will be dependent on the size of the cells, effectively sorting them through separate outlets.

The researchers found they could improve the sorter’s operation by running it twice, first at a lower speed so larger cells stick to the walls and smaller cells are sorted out, then at a higher speed to sort out larger cells.

In a sense, the device operates like a centrifuge, but the microfluidic sorter does not require human intervention to pick out sorted cells, Han adds.

The researchers showed that their device could remove about 50 percent of the larger cells with one pass. They conducted experiments to confirm that the larger cells they removed were, in fact, associated with higher tumor risk.

“While we can’t remove 100 percent of these cells, we still believe this is going to reduce the risk significantly. Hopefully, the original cell type is good enough that we don’t have too many undifferentiated cells. Then this process could make these cells even safer,” he says.

Importantly, the low-cost microfluidic sorter, which can be produced at scale with standard manufacturing techniques, does not use any type of filtration. Filters can become clogged or break down, so a filter-free device can be used for a much longer time.

Now that they have shown success at a small scale, the researchers are embarking on larger studies and animal models to see if the purified cells function better in vivo.

Nondifferentiated cells can become tumors, but they can have other random effects in the body, so removing more of these cells could boost the efficacy of cell therapies, as well as improve safety.

“If we can convincingly demonstrate these benefits in vivo, the future might hold even more exciting applications for this technique,” Han says.

This research is supported, in part, by the National Research Foundation of Singapore and the Singapore-MIT Alliance for Research and Technology.

Technique could improve the sensitivity of quantum sensing devices

In quantum sensing, atomic-scale quantum systems are used to measure electromagnetic fields, as well as properties like rotation, acceleration, and distance, far more precisely than classical sensors can. The technology could enable devices that image the brain with unprecedented detail, for example, or air traffic control systems with precise positioning accuracy.

As many real-world quantum sensing devices are emerging, one promising direction is the use of microscopic defects inside diamonds to create “qubits” that can be used for quantum sensing. Qubits are the building blocks of quantum devices.

Researchers at MIT and elsewhere have developed a technique that enables them to identify and control a greater number of these microscopic defects. This could help them build a larger system of qubits that can perform quantum sensing with greater sensitivity.

Their method builds off a central defect inside a diamond, known as a nitrogen-vacancy (NV) center, which scientists can detect and excite using laser light and then control with microwave pulses. This new approach uses a specific protocol of microwave pulses to identify and extend that control to additional defects that can’t be seen with a laser, which are called dark spins.

The researchers seek to control larger numbers of dark spins by locating them through a network of connected spins. Starting from this central NV spin, the researchers build this chain by coupling the NV spin to a nearby dark spin, and then use this dark spin as a probe to find and control a more distant spin which can’t be sensed by the NV directly. The process can be repeated on these more distant spins to control longer chains.

“One lesson I learned from this work is that searching in the dark may be quite discouraging when you don’t see results, but we were able to take this risk. It is possible, with some courage, to search in places that people haven’t looked before and find potentially more advantageous qubits,” says Alex Ungar, a PhD student in electrical engineering and computer science and a member of the Quantum Engineering Group at MIT, who is lead author of a paper on this technique, which is published today in PRX Quantum.

His co-authors include his advisor and corresponding author, Paola Cappellaro, the Ford Professor of Engineering in the Department of Nuclear Science and Engineering and professor of physics; as well as Alexandre Cooper, a senior research scientist at the University of Waterloo’s Institute for Quantum Computing; and Won Kyu Calvin Sun, a former researcher in Cappellaro’s group who is now a postdoc at the University of Illinois at Urbana-Champaign.

Diamond defects

To create NV centers, scientists implant nitrogen into a sample of diamond.

But introducing nitrogen into the diamond creates other types of atomic defects in the surrounding environment. Some of these defects, including the NV center, can host what are known as electronic spins, which originate from the valence electrons around the site of the defect. Valence electrons are those in the outermost shell of an atom. A defect’s interaction with an external magnetic field can be used to form a qubit.

Researchers can harness these electronic spins from neighboring defects to create more qubits around a single NV center. This larger collection of qubits is known as a quantum register. Having a larger quantum register boosts the performance of a quantum sensor.

Some of these electronic spin defects are connected to the NV center through magnetic interaction. In past work, researchers used this interaction to identify and control nearby spins. However, this approach is limited because the NV center is only stable for a short amount of time, a principle called coherence. It can only be used to control the few spins that can be reached within this coherence limit.

In this new paper, the researchers use an electronic spin defect that is near the NV center as a probe to find and control an additional spin, creating a chain of three qubits.

They use a technique known as spin echo double resonance (SEDOR), which involves a series of microwave pulses that decouple an NV center from all electronic spins that are interacting with it. Then, they selectively apply another microwave pulse to pair the NV center with one nearby spin.

Unlike the NV, these neighboring dark spins can’t be excited, or polarized, with laser light. This polarization is a required step to control them with microwaves.

Once the researchers find and characterize a first-layer spin, they can transfer the NV’s polarization to this first-layer spin through the magnetic interaction by applying microwaves to both spins simultaneously. Then once the first-layer spin is polarized, they repeat the SEDOR process on the first-layer spin, using it as a probe to identify a second-layer spin that is interacting with it.

Controlling a chain of dark spins

This repeated SEDOR process allows the researchers to detect and characterize a new, distinct defect located outside the coherence limit of the NV center. To control this more distant spin, they carefully apply a specific series of microwave pulses that enable them to transfer the polarization from the NV center along the chain to this second-layer spin.

“This is setting the stage for building larger quantum registers to higher-layer spins or longer spin chains, and also showing that we can find these new defects that weren’t discovered before by scaling up this technique,” Ungar says.

To control a spin, the microwave pulses must be very close to the resonance frequency of that spin. Tiny drifts in the experimental setup, due to temperature or vibrations, can throw off the microwave pulses.

The researchers were able to optimize their protocol for sending precise microwave pulses, which enabled them to effectively identify and control second-layer spins, Ungar says.

“We are searching for something in the unknown, but at the same time, the environment might not be stable, so you don’t know if what you are finding is just noise. Once you start seeing promising things, you can put all your best effort in that one direction. But before you arrive there, it is a leap of faith,” Cappellaro says.

While they were able to effectively demonstrate a three-spin chain, the researchers estimate they could scale their method to a fifth layer using their current protocol, which could provide access to hundreds of potential qubits. With further optimization, they may be able to scale up to more than 10 layers.

In the future, they plan to continue enhancing their technique to efficiently characterize and probe other electronic spins in the environment and explore different types of defects that could be used to form qubits.

This research is supported, in part, by the U.S. National Science Foundation and the Canada First Research Excellence Fund.

3 Questions: The Climate Project at MIT

MIT is preparing a major campus-wide effort to develop technological, behavioral, and policy solutions to some of the toughest problems now impeding an effective global climate response. The Climate Project at MIT, as the new enterprise is known, includes new arrangements for promoting cross-Institute collaborations and new mechanisms for engaging with outside partners to speed the development and implementation of climate solutions.

MIT News spoke with Richard K. Lester, MIT’s vice provost for international activities, who has helped oversee the development of the project.

Q: What is the Climate Project at MIT?

A: In her inaugural address last May, President Kornbluth called on the MIT community to join her in a “bold, tenacious response” to climate change. The Climate Project at MIT is a response to that call. It aims to mobilize every part of MIT to develop, deliver, and scale up practical climate solutions, as quickly as possible.

At MIT, well over 300 of our faculty are already working with their students and research staff members on different aspects of the climate problem. Almost all of our academic departments and more than a score of our interdepartmental labs and centers are involved in some way. What they are doing is remarkable, and this decentralized structure reflects the best traditions of MIT as a “bottom up,” entrepreneurial institution. But, as President Kornbluth said, we must do much more. We must be bolder in our research choices and more creative in how we organize ourselves to work with each other and with our partners. The purpose of the Climate Project is to support our community’s efforts to do bigger things faster in the climate domain. We will have succeeded if our work changes the trajectory of global climate outcomes for the better.

I want to be clear that the clay is still wet here. The Climate Project will continue to take shape as more members of the MIT community bring their excellence, their energy, and their ambition to bear on the climate challenge. But I believe we have a vision and a framework for accelerating and amplifying MIT’s real-world climate impact, and I know that President Kornbluth is eager to share this progress report with the MIT community now to convey the breadth and ambition of what we’re planning.

Q: How will the project be organized?

A: The Climate Project will have three core components: the Climate Missions; their offshoots, the Climate Frontier Projects; and Climate HQ. A new vice president for climate will lead the enterprise.

Initially there will be six missions, which you can read about in the plan. Each will address a different domain of climate impact where new solutions are required and where a critical mass of research excellence exists at MIT. One such mission, of course, is to decarbonize energy and industry, an area where we estimate that about 150 of our faculty are already working.

The mission leaders will build multidisciplinary problem-solving communities reaching across the Institute and beyond. Each of these will be charged with roadmapping and assessing progress toward its mission, identifying critical gaps and bottlenecks, and launching applied research projects to accelerate progress where the MIT community and our partners are well-positioned to achieve impactful results. These projects — the climate frontier projects — will benefit from active, professional project management, with clear metrics and milestones. We are in a critical decade for responding to climate change, so it’s important that these research projects move quickly, with an eye on producing real-world results.

The new Climate HQ will drive the overall vision for the Climate Project and support the work of the missions. We’ve talked about a core focus on impact-driven research, but much is still unknown about the Earth’s physical and biogeochemical systems, and there is also much to be learned about the behavior of the social and political systems that led us to the very difficult situation the world now faces. Climate HQ will support fundamental research in the scientific and humanistic disciplines related to climate, and will promote engagement between these disciplines and the missions. We must also advance climate-related education, led by departments and programs, as well as policy work, public outreach, and more, including an MIT-wide student-centric Climate Corps to elevate climate-related, community-focused service in MIT’s culture.

Q: Why are partners a key part of this project?

A: It is important to build strong partners right from the very start for our innovations, inventions, and discoveries to have any prospect of achieving scale. And in many cases, with climate change, it’s all about scale.

One of the aims of this initiative is to strengthen MIT’s climate “scaffolding” — the people and processes connecting what we do on campus to the practical world of climate impact and response. We can build on MIT’s highly developed infrastructure for translation, innovation, and entrepreneurship, even as we promote other important pathways to scale involving communities, municipalities, and other not-for-profit organizations. Working with all these different organizations will help us build a broad infrastructure to help us get traction in the world. On a related note, the Sloan School of Management will be sharing details in the coming days of an exciting new effort to enhance MIT’s contributions in the climate policy arena.

MIT is committing $75 million, including $25 million from Sloan, at the outset of the project. But we anticipate developing new partnerships, including philanthropic partnerships, to increase that scope dramatically.